Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Leber, B; Stadlbauer, V; Stiegler, P; Stanzer, S; Mayrhauser, U; Koestenbauer, S; Leopold, B; Sereinigg, M; Puntschart, A; Stojakovic, T; Tscheliessnigg, KH; Oettl, K.
Effect of oxidative stress and endotoxin on human serum albumin in brain-dead organ donors.
Transl Res. 2012; 159(6):487-496 Doi: 10.1016/j.trsl.2011.12.005
Web of Science PubMed FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Leber Bettina
Stadlbauer-Köllner Vanessa
Stiegler Philipp
Co-Autor*innen der Med Uni Graz
Köstenbauer Sonja
Leopold-Posch Barbara
Mayrhauser Ursula
Öttl Karl
Puntschart Andreas Jürgen
Sereinigg Michael
Stanzer Stefanie
Stojakovic Tatjana
Tscheliessnigg Karlheinz

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

Albumin, among other molecules, binds and detoxifies endotoxin in healthy people. Oxidative stress leads to protein oxidation and thus to the impaired binding properties of albumin. This property, in combination with increased gut permeability, leads to the appearance of endotoxin in the systemic circulation and to impaired organ function. We hypothesize that these processes occur in the serum of brain-dead organ donors. Endotoxin was determined with an adapted Limulus amoebocyte lysate assay. The albumin fractions and binding capacity were determined by high-performance liquid chromatography (HPLC). FlowCytomix (eBioscience, San Diego, Calif) was used to determine the cytokine levels. Carbonylated proteins (CPs) and myeloperoxidase (MPO) were measured by an enzyme-linked immunosorbent assay (ELISA). Eighty-four brain-dead organ donors were enrolled and categorized by the duration of intensive care unit (ICU) stay. The albumin-binding capacity for dansylsarcosine was reduced in brain-dead patients compared with controls. Endotoxin positivity in 16.7% of donors was associated with decreased binding capacity in donors and worse survival of recipients. The CP and MPO levels of organ donors were significantly higher than in healthy controls. The durations of ICU stay increased albumin oxidation. In addition, interleukin-6 (IL-6), IL-8, IL-10, and IL-1β levels were increased in patients, whereas the interferon-γ (IFN-γ) levels were within the normal range. We conclude that oxidative stress and systemic endotoxemia are present in brain-dead organ donors, which might affect recipient survival. High endotoxin levels might be caused by increased gut permeability and decreased binding capacity of albumin influenced not just by higher albumin oxidation.
Find related publications in this database (using NLM MeSH Indexing)
Adolescent -
Adult -
Aged -
Brain Death - blood
Cytokines - blood
Endotoxins - blood
Female -
Humans -
Intensive Care -
Interleukins - blood
Kaplan-Meier Estimate -
Male -
Middle Aged -
Oxidative Stress -
Peroxidase - blood
Protein Binding -
Protein Carbonylation -
Retrospective Studies -
Serum Albumin - metabolism
Time Factors -
Tissue Donors -
Tissue and Organ Harvesting -
Translational Medical Research -
Transplants -
Young Adult -

© Med Uni Graz Impressum