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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Raggam, RB; Salzer, HJF; Marth, E; Heiling, B; Paulitsch, AH; Buzina, W.
Molecular detection and characterisation of fungal heat shock protein 60
Mycoses. 2011; 54(5):e394-e399 Doi: 10.1111/j.1439-0507.2010.01933.x
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Führende Autor*innen der Med Uni Graz

Buzina Walter

Raggam Reinhard Bernd

Salzer Helmut J. F.

Co-Autor*innen der Med Uni Graz

Kölli Bettina

Marth Egon

Paulitsch-Fuchs Astrid Helga

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Abstract:

Heat shock proteins (Hsp) are highly conserved molecules, which are both constitutively expressed and up-regulated in response to various stress conditions. In particular, fungal Hsp60 can act as immunodominant antigens and facilitate powerful immunological properties. A possible cellular heat shock response was investigated in eight fungi (Aspergillus fumigatus, Aspergillus terreus, Penicillium chrysogenum, Cladosporium cladosporioides, Scedosporium apiospermum, Trichophyton mentagrophytes, Candida albicans and Saccharomyces cerevisiae). Fully automated RNA extraction was followed by quantitative real-time RT-PCR targeting fungus-specific Hsp60 mRNA and sequencing of the amplicon. Levels of temperature-dependent gene expression were evaluated and rates of similarity and identity were compared. While Hsp60 mRNA was constitutively expressed in all the samples tested, a temperature-dependent induction was not shown in C. cladosporioides. In the 80-amino acid fragment from the hypothetical protein, 66% of the amino acids were identical, 20% showed a conserved and 8% a semi-conserved substitution. Our findings should contribute to a better understanding of host-pathogen relationship and suggest that fungal Hsp60 under temperature-related stress conditions might act as an immunogenic trigger in orchestrating fungi-related diseases.

Find related publications in this database (using NLM MeSH Indexing)

Amino Acid Substitution -

Chaperonin 60 - genetics

Fungal Proteins - genetics

Fungi - radiation effects

Gene Expression Profiling -

Gene Expression Regulation, Fungal -

Hot Temperature -

Real-Time Polymerase Chain Reaction -

Reverse Transcriptase Polymerase Chain Reaction -

Sequence Analysis, DNA -

Sequence Homology, Amino Acid -

Stress, Physiological -

Find related publications in this database (Keywords)

Heat shock protein

fungal Hsp60

heat shock response

real-time RT-PCR

similarity and identity

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