Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kiessling, M; Gindlhuber, J; Sintou, A; Matzer, I; Radulović, S; Trummer-Herbst, V; Ajdari, A; Voglhuber-Höller, J; Holzer, M; Rodriguez, TA; Leitinger, G; Zirlik, A; Bers, DM; Sattler, S; Ljubojevic-Holzer, S.
A transmission electron microscopy platform for assessing mitochondrial and nuclear architecture in cardiomyocytes.
Cell Rep Methods. 2025; 101212
Doi: 10.1016/j.crmeth.2025.101212
PubMed
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- Leading authors Med Uni Graz
- Holzer Senka
- Kießling Mara Luisa
- Sattler Susanne
- Co-authors Med Uni Graz
- Ajdari Andonita
- Gindlhuber Jürgen
- Holzer Michael
- Leitinger Gerd
- Matzer Ingrid
- Radulovic Snjezana
- Trummer-Herbst Viktoria
- Voglhuber Julia
- Zirlik Andreas
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- Abstract:
- Mitochondria are central to cardiomyocyte function, and their spatial organization regulates nuclear signaling and gene transcription, holding potential for novel cardioprotective interventions. We developed a transmission electron microscopy platform optimized for resolving mitochondrial subpopulations and nuclear architecture in adult cardiomyocytes. This approach reliably captures longitudinal sections containing the center of the nucleus and perinuclear regions, enabling consistent imaging of subcellular nanostructures, assessment of pharmacological effects within the same organism, and visualization of extracellular vesicles carrying dysfunctional mitochondria. Integrated with an analysis workflow employing machine learning-based segmentation for annotation, the method allows automated quantification of mitochondrial and nuclear architecture and positioning. Using Drp1-deficient mice with impaired mitochondrial fission, we demonstrate this tool's ability to uncover nanoscale remodeling of mitochondria and nuclei under stress. Our platform overcomes challenges in electron microscopy analysis, providing a powerful resource to interrogate mitochondrial-nuclear dynamics in cardiac (patho)physiology. These insights will inform therapeutic targeting of bioenergetic failure.