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Donnerer, J; Liebmann, I.
pERK1/2 immunofluorescence in rat dorsal horn and paraventricular nucleus neurons as a marker for sensitization and inhibition in the pain pathway.
Tissue Cell. 2015; 47(1):55-60 Doi: 10.1016/j.tice.2014.11.003
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Leading authors Med Uni Graz: Donnerer Josef
Co-authors Med Uni Graz: Liebmann Ingrid
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Abstract:: The aim of the present study was to investigate whether the phosphorylation of ERK1/2 in the rat lumbar dorsal horn and in the parvocellularis part of the paraventricular nucleus can be used to visualize neuronal activity. pERK1/2 fluorescence-immunohistochemistry is specifically suited to mirror neuronal activity in the pain pathway following an acute noxious stimulation. The rat hind paw was either stimulated by noxious heat or by a sequence of mustard oil and noxious heat. Two and 10 min after the thermal stimulation a 3-4-fold increase in cells with pERK1/2 immunoreactivity was observed in lamina I/II of the L3-L5 dorsal horn. The combination of mustard oil with heat led to a 5-6-fold increase in the pERK1/2 signal. The pERK1/2 immunoreactivity in the parvocellularis part of the paraventricular nucleus increased by 2-fold following the heat stimulus, with no further increase following the sequential mustard oil and heat stimulus. A pretreatment with the opioid analgesic morphine or the NMDA antagonist MK-801 markedly attenuated ERK1/2 phosphorylation in both areas of the pain pathway. The present findings support the concept that the pERK1/2 immunofluorescence signal can be used as a quantitative marker for sensitization or inhibition in the pain pathway at spinal and hypothalamic level. Copyright © 2014 Elsevier Ltd. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Fluorescent Antibody Technique -; Hot Temperature -; Male -; Mitogen-Activated Protein Kinase 3 - metabolism; Morphine - administration & dosage; Mustard Plant - toxicity; Neurons - enzymology; Neurons - pathology; Pain - chemically induced; Pain - genetics; Pain - pathology; Paraventricular Hypothalamic Nucleus - enzymology; Paraventricular Hypothalamic Nucleus - pathology; Phosphorylation -; Plant Oils - toxicity; Rats -; Rats, Sprague-Dawley -; Spinal Cord Dorsal Horn - enzymology; Spinal Cord Dorsal Horn - pathology
Find related publications in this database (Keywords): ERK1/2 phosphorylation; Nociception; Opioid analgesic; NMDA antagonist; Spinal cord; Hypothalamus