Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Siegemund, T; Paulke, BR; Schmiedel, H; Bordag, N; Hoffmann, A; Harkany, T; Tanila, H; Kacza, J; Härtig, W.
Thioflavins released from nanoparticles target fibrillar amyloid beta in the hippocampus of APP/PS1 transgenic mice.
Int J Dev Neurosci. 2006; 24(2-3):195-201 Doi: 10.1016/j.ijdevneu.2005.11.012
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Co-authors Med Uni Graz

Bordag Natalie

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Abstract:

For the delivery of drugs into the brain, the use of nanoparticles as carriers has been described as a promising approach. Here, we prepared nanoparticles as carriers for the model drugs thioflavin T and thioflavin S that bind fibrillar amyloid beta peptides (Abeta). These polymer colloids are composed of a polystyrene core and a degradable PBCA [poly(butyl-2-cyanoacrylate)] shell with a diameter of 90-100nm as shown by dynamic light scattering. Fluorescence spectrophotometric analysis revealed that encapsulated thioflavin T exhibited significantly stronger fluorescence than the free fluorophore. The enzymatic degradation of core-shell nanoparticles, as required in vivo, was shown after their treatment with porcine liver esterase, a non-specific esterase, in vitro. Shells of nanoparticles were dose-dependently degraded while their polystyrene cores remained intact. In the cortices of 7-14 months old APP/PS1 mice with age-dependent beta-amyloidosis, thioflavins selectively targeted fibrillar Abeta after biodegradation-induced release from their nanoparticulate carriers upon intracerebral injection. Collectively, our data suggest that core-shell nanoparticles with controlled degradation in vivo can become versatile tools to trace and clear Abeta in the brain.

Find related publications in this database (using NLM MeSH Indexing)

Alzheimer Disease - drug therapy Alzheimer Disease - metabolism Alzheimer Disease - pathology

Amyloid - metabolism

Amyloid beta-Protein Precursor - genetics Amyloid beta-Protein Precursor - metabolism

Animals -

Disease Models, Animal -

Dose-Response Relationship, Drug -

Drug Delivery Systems -

Hippocampus - drug effects Hippocampus - metabolism

Mice -

Mice, Transgenic -

Nanostructures -

Neurofibrillary Tangles - drug effects Neurofibrillary Tangles - metabolism

Protein Binding - drug effects

Thiazoles - administration & dosage

Time Factors -

Find related publications in this database (Keywords)

Alzheimer's disease

drug delivery

drug targeting

dynamic light scattering

senile plaque

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